Validation of microarray data. Total RNA was extracted from four independent HeLa cell infections with indicated shRNA vectors for five days including aliquots of RNA for microarray analysis. Quantitative real-time RT-PCR was carried out to measure the expression level of indicated mRNA. The fold-change was calculated and represents the change in transcript levels in shRNA T2 or T1 infected cells relative to shRNA MM treated cells after normalization to housekeeping gene, GAPDH. Average fold-change from the four independent infections is presented. * p < 0.05, ** p < 0.005, *** p < 0.0005 in a paired t-test.