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Table 1 Reaction inhibition, fluorescent signal, and melting temperature data: 16S amplicon

From: SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR

Dye Optimal Concentration C t : 0.64 μM
(0.32×)
C t : 2 μM
(1×)
C t : 10 μM
(5×)
C t : 20 μM
(10×)
Maximum Fluorescence T m shift
slope (R 2 )
SYBR Green* 0.64 μM (0.32×) 27.4 ± 0.78 no Ct no Ct no Ct 1.00 5.89, × conc.
2.95, μM conc.
(0.86)
EvaGreen* 31.54 ± 0.21 29.98 ± 0.50 28.60 ± 0.50 not determined 1.03 0.50 (0.94)
Green*        
SYTO 11 2 μM 30.8 ± 1.26 29.9 ± 1.06 33.7 ± 2.58 no Ct 0.83 0.41 (0.96)
SYTO 13 10 μM 31.8 ± 1.06 30.5 ± 1.16 29.1 ± 0.95 29.9 ± 1.11 0.85 0.15 (0.92)
SYTO 16 10 μM 29.8 ± 0.95 28.5 ± 0.92 27.7 ± 1.04 30.3 ± 1.47 0.99 0.17 (0.93)
SYTO 21 0.64 μM 28.5 ± 0.98 32.94 ± 3.7 no Ct no Ct 0.79 1.73 (0.63)
SYTO 24 0.64 μM 31.0 ± 0.0 no Ct no Ct no Ct 0.98 not determined
Orange*        
SYTO 80 20 μM 33.9 ± 0.57 32.4 ± 0.09 30.27 ± 0.01 29.4 ± 0.09 0.54 0.018 (0.33)
SYTO 81 20 μM 40.13 ± 0.0 35.7 ± 0.55 32.5 ± 0.10 32.0 ± 0.03 0.13 -0.002 (0.003)
SYTO 82 20 μM 31.8 ± 0.37 31.25 ± 0.06 29.4 ± 0.01 28.7 ± 0.08 0.77 0.040 (0.59)
SYTO 83 20 μM 39.6 ± 2.09 35.2 ± 0.77 32.7 ± 0.41 32.4 ± 0.17 0.12 0.029 (0.38)
Red**        
SYTO 17 20 μM no Ct no Ct 32.9 30.9 0.05 -0.001 (0.009)
SYTO 59 2 μM 28.9 28.0 28.5 37.2 0.29 0.16 (0.96)
SYTO 60 2 μM 29.5 29.37 no Ct no Ct 0.16 0.24 (0.78)
SYTO 61 10 μM 32.0 29.86 27.76 31.55 0.17 0.06 (0.91)
SYTO 62 0.64 μM 28.8 30.75 no Ct no Ct 0.18 0.26 (0.99)
SYTO 63 2 μM 28.9 28.6 43.0 no Ct 0.20 0.12 (0.32)
SYTO 64 2 μM 34.8 31.7 31.7 34.0 0.15 0.012 (0.27)
  1. No fluorescent signals were obtained for SYTO dyes 12, 14, 25, 84, or 85
  2. *Ct values are average ± SD for at 2-3 independent experiments
  3. **Ct values from one independent experiment, no SD