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Table 2 Reaction inhibition, fluorescent signal, and melting temperature data: tropomyosin amplicon

From: SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR

Dye Optimal Concentration C t : 0.64 μM
(0.32×)
C t : 2 μM
(1×)
C t : 10 μM
(5×)
C t : 20 μM
(10×)
Maximum Fluorescence T m shift
slope (R 2 )
SYBR Green* 0.64 μM 33.00 ± 0.10 35.07 ± 1.10 no Ct no Ct 1.00 3.99, × conc.
1.99, μM conc.
(0.97)
EvaGreen* 36.37 ± 0.48 35.04 ± 0.35 35.15 ± 1.13 not determined 0.91 0.36 (0.98)
Green*        
SYTO 11 2 μM 38.02 ± 0.97 36.42 ± 0.84 38.17 ± 1.03 42.27 ± 0 0.51 -0.20 (0.12)
SYTO 13 10 μM 37.58 ± 1.33 36.10 ± 1.32 36.06 ± 1.55 36.45 ± 2.06 0.87 0.001 (0.00)
SYTO 16 10 μM 37.00 ± 1.86 34.20 ± 1.05 34.04 ± 0.96 34.97 ± 0.53 1.07 0.030 (0.004)
SYTO 21 0.64 μM 34.55 ± 1.07 35.90 ± 0.83 no Ct no Ct 0.82 0.62 (0.03)
SYTO 24 0.64 μM 33.50 ± 0.01 no Ct no Ct no Ct 1.02 not determined
Orange*        
SYTO 80 20 μM 39.16 ± 2.44 37.33 ± 1.86 35.84 ± 0.02 34.78 ± 0.08 0.40 0.048 (0.008)
SYTO 81 20 μM 43.89 ± 0.10 40.62 ± 1.39 37.02 ± 0.39 36.45 ± 0.21 0.11 0.026 (0.002)
SYTO 82 20 μM 37.18 ± 1.85 35.59 ± 0.95 33.50 ± 0.11 33.44 ± 0.51 0.61 -0.17 (0.10)
SYTO 83 20 μM 42.20 ± 0.01 39.28 ± 0.99 37.04 ± 0.13 35.92 ± 0.93 0.10 0.037 (0.0051)
Red*        
SYTO 17 20 μM no Ct 42.26 ± 0.02 36.18 ± 0.14 35.32 ± 0.41 0.03 0.0009 (0.0005)
SYTO 59 10 μM 35.63 ± 0.09 35.15 ± 1.50 33.70 ± 0.10 35.53 ± 0.53 0.20 0.11 (0.83)
SYTO 60 2 μM 34.85 ± 0.41 34.29 ± 0.31 41.09 ± 1.97 no Ct 0.13 0.064 (0.10)
SYTO 61 10 μM 37.45 ± 1.68 35.38 ± 0.57 34.14 ± 0.98 35.00 ± 1.19 0.14 0.043 (0.58)
SYTO 62 0.64 μM 33.98 ± 0.42 33.69 ± 0.75 no Ct no Ct 0.17 0.035 (0.008)
SYTO 63 2 μM 34.78 ± 0.41 34.50 ± 0.31 37.07 ± 0.07 no Ct 0.12 0.24 (0.85)
SYTO 64 2 μM 36.22 ± 0.10 36.68 ± 1.62 36.31 ± 1.05 36.48 ± 0.95 0.05 -0.004 (0.013)
  1. No fluorescent signals were obtained for SYTO dyes 12, 14, 25, 84, or 85
  2. *Ct values are average ± SD for at 2-3 independent experiments