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Table 3 Reaction efficiency data (16S amplicon)

From: SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR

Dye Concentration R 2 Slope Efficiency
SYBR Green 0.32 × (0.64 μM) 0.985 -3.40 97%
SYBR Green 1× (2 μM) 0.395 -2.13 195%
EvaGreen 0.995 -3.30 101%
EvaGreen 0.999 -3.46 95%
SYTO 13 5 μM 0.993 -3.42 96%
SYTO 13 10 μM 0.993 -3.44 95%
SYTO 16 5 μM 0.983 -3.30 101%
SYTO 16 10 μM 0.992 -3.43 96%
SYTO 80 10 μM 0.992 -3.42 96%
SYTO 80 20 μM 0.986 -3.41 96%
SYTO 82 10 μM 0.998 -3.41 96%
SYTO 82 20 μM 0.996 -3.37 98%