Figure 6From: Cross-species protein sequence and gene structure prediction with fine-tuned Webscipio 2.0 and ScipioReconstructing genes on chromosome assemblies. The scheme shows an example of the search for the rat homolog (target sequence) of the human Kif5C kinesin motor protein. The C-terminal about 25 amino acids of the rat Kif5C homolog are missing in the respective chromosome assembly. Using Scipio v1.0 a very short identical stretch of four amino acids, found on a different chromosome, has artificially been added to the 3'-end of the gene generating an "intron" of millions of base pairs (Note the scale of the introns!). The new parameter --single_target_hits now prevents this mis-assembly. The colour coding of the scheme is the same as in Figure 3.Back to article page