Reconstructing genes on chromosome assemblies. The scheme shows an example of the search for the rat homolog (target sequence) of the human Kif5C kinesin motor protein. The C-terminal about 25 amino acids of the rat Kif5C homolog are missing in the respective chromosome assembly. Using Scipio v1.0 a very short identical stretch of four amino acids, found on a different chromosome, has artificially been added to the 3'-end of the gene generating an "intron" of millions of base pairs (Note the scale of the introns!). The new parameter --single_target_hits now prevents this mis-assembly. The colour coding of the scheme is the same as in Figure 3.