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Table 3 Details of the mutations found by TILLING in the gene targets screened in the two EMS-mutagenized melon populations, Piel de Sapo (PS) and Charentais (CH)

From: Towards a TILLING platform for functional genomics in Piel de Sapo melons

Gene/population Amplicon size (bp) Identified mutants 1 Exon/Intron Position AA change M2 Family M2 segregation (WT/H/M)2 SIFT score
Cm-eIF4E/PS 500 1 1 Exon 1 A 74 G R 25 K F2036 9/7/8 0.00
  1169 3 2 Exon 2 G 385 A E 129 K F57 5/3/0 0.00
    3 Exon 2 G 465 A T 155 T F1880   
    4 Intron 2 G/C   F959   
Cm-eIF4E/CH 500 3 5 Exon 1 G 289 A E 97 K 53   0.02
    6 Exon 1 C 294 T F 98 F 2532   
    7 Intron 1 G/A   1420   
  1169 11 8 Exon 2 C 401 T A 134 V 1229   0.10
    9 Exon 2 G 407 A G 136 E 444   0.00
    10 Intron 2 C/A   1287   
    11 Intron 2 C/T   1115   
    12 Intron 3 G/A first nt of intron splicing 1667   
    13 Intron 3 C/T   2646   
    14 Intron 3 C/T   1233   
    15 Exon 4 G 616 A E 206 K 2060   0.09
    16 Intron 4 C/T   2035   
    17 3'UTR T/C   1368   
    18 3'UTR G/A   2163   
      222 TGA     
CmeIF(iso)4E/PS 1014 0 -       
CmeIF(iso)4E/CH 1014 1 19 Exon 1 G 128 A R 43 K M1587   1.00
Cm-PDS/PS 1188 2 20 Intron 1 C/T   F1151 4/7/4  
    21 Exon 2 C 560 T S 227 F C384 6/9/0 0.00
Cm-PDS/CH 1188 4 22 Exon 1 C 395 T S 172 F 1182   0.01
    23 Intron 1 C/T   2495   
    24 Intron 1 T/C   1460   
    25 Intron 1 C/T   1226   
Cm-ACO1/PS 933 0 -       
  933 1 26 Exon 4 C 728 T T 243 I C142 10/6/3 1.00
Cm-NOR/PS 1272 0        
Cm-DET1/PS 1185 1 27 Exon 2 T 402 A L 135 L I76   
  776 2 28 Exon 4 G 760 A V 255 I F201 2/11/2 0.37
    29 Exon 4 C 848 T S 284 L F1876 2/4/3 0.25
  1232 2 30 Intron 6 G 2981 A   F1248   
    31 Intron 7 C 3211 T   C487   
Cm-DHS/PS 578 1 32 Exon 1 G 184 A V 62 I F 1728 13/20/0 0.58
  754 1 33 Exon 3 G 610 A V 204 I C348 2/6/0 0.27
  1. 1Number of mutations corresponds to those represented in Figures 1 and 2
  2. 2 For some mutations, it was difficult to obtain homozygous MM plants as seedlings showed altered development.