Table 3 Details of the mutations found by TILLING in the gene targets screened in the two EMS-mutagenized melon populations, Piel de Sapo (PS) and Charentais (CH)

Cm-eIF4E/PS

500

1

1

Exon 1

A 74 G

R 25 K

F2036

0.00

1169

3

2

Exon 2

G 385 A

E 129 K

F57

0.00

3

Exon 2

G 465 A

T 155 T

F1880

4

Intron 2

G/C

F959

Cm-eIF4E/CH

500

3

5

Exon 1

G 289 A

E 97 K

53

0.02

6

Exon 1

C 294 T

F 98 F

2532

7

Intron 1

G/A

1420

1169

11

8

Exon 2

C 401 T

A 134 V

1229

0.10

9

Exon 2

G 407 A

G 136 E

444

0.00

10

Intron 2

C/A

1287

11

Intron 2

C/T

1115

12

Intron 3

G/A first nt of intron

splicing

1667

13

Intron 3

C/T

2646

14

Intron 3

C/T

1233

15

Exon 4

G 616 A

E 206 K

2060

0.09

16

Intron 4

C/T

2035

17

3'UTR

T/C

1368

18

3'UTR

G/A

2163

222 TGA

CmeIF(iso)4E/PS

1014

0

-

CmeIF(iso)4E/CH

1014

1

19

Exon 1

G 128 A

R 43 K

M1587

1.00

Cm-PDS/PS

1188

2

20

Intron 1

C/T

F1151

21

Exon 2

C 560 T

S 227 F

C384

0.00

Cm-PDS/CH

1188

4

22

Exon 1

C 395 T

S 172 F

1182

0.01

23

Intron 1

C/T

2495

24

Intron 1

T/C

1460

25

Intron 1

C/T

1226

Cm-ACO1/PS

933

0

-

933

1

26

Exon 4

C 728 T

T 243 I

C142

1.00

Cm-NOR/PS

1272

0

Cm-DET1/PS

1185

1

27

Exon 2

T 402 A

L 135 L

I76

776

2

28

Exon 4

G 760 A

V 255 I

F201

0.37

29

Exon 4

C 848 T

S 284 L

F1876

0.25

1232

2

30

Intron 6

G 2981 A

F1248

31

Intron 7

C 3211 T

C487

Cm-DHS/PS

578

1

32

Exon 1

G 184 A

V 62 I

F 1728

0.58

754

1

33

Exon 3

G 610 A

V 204 I

C348

0.27