Co-immunoprecipitation of CD99 with other membrane proteins. Immunoprecipitation of biotinylated Jurkat cell lysates using mild detergent 1% Brij-58 (A) or raft-disrupting detergent 1% Lauryl maltoside (B). Cell lysates were precipitated using isotype matched control 4G2 mAb (lane 1) and anti-CD99 mAb MT99/3 (lane 2). Electrophoresis was performed under reducing conditions. The positions of molecular mass markers are indicated on the left in kDa. The positions of CD99 molecules are indicated.