Figure 1From: Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv)Scheme of the PCR for scFv assembly. A: Amplification of VH and VL chains using primers (arrows) containing a region complementary to the linker (dotted line). B: Amplification of VL chain connecting to the linker, generating the LINKER-VL fragment. C: Amplification and linkage of VH to the LINKER-VL and anti-intimin scFv amplification.Back to article page