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Figure 6 | BMC Research Notes

Figure 6

From: Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv)

Figure 6

Functional assay of anti-intimin scFv by indirect sandwich ELISA. Microplates were coated with a serial dilution of anti-intimin scFv, followed by incubation with 2 μg/mL of purified intimin and a serial dilution of rabbit anti-intimin IgG enriched fraction antibody. The graph shows the best fit titration curve of 275 ng of anti-intimin scFv reacting with 2 μg of purified intimin in which the optimal absorbance was defined by 7.5 μg rabbit anti-intimin IgG enriched fraction antibody (*).

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