Figure 7From: Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv)Functional assay of scFv-intimin by immunofluorescence. A. EPEC E2348/69 strain permeabilized with 4% Triton X-100 followed by incubation with 4.4 μg of anti-intimin scFv. The reaction was developed using anti-His antibody and anti-mouse IgG conjugated to FITC. B. E. coli K12 JM109 (negative control) assayed as described for A.Back to article page