TY - JOUR AU - Pellino, Marco AU - Sharbel, Timothy F. AU - Mau, Martin AU - Amiteye, Samuel AU - Corral, José María PY - 2011 DA - 2011/08/19 TI - Selection of reference genes for quantitative real-time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera JO - BMC Research Notes SP - 303 VL - 4 IS - 1 AB - Apomixis, a natural form of asexual seed production in plants, is considered to have great biotechnological potential for agriculture. It has been hypothesised that de-regulation of the sexual developmental pathway could trigger apomictic reproduction. The genus Boechera represents an interesting model system for understanding apomixis, having both sexual and apomictic genotypes at the diploid level. Quantitative qRT-PCR is the most extensively used method for validating genome-wide gene expression analyses, but in order to obtain reliable results, suitable reference genes are necessary. In this work we have evaluated six potential reference genes isolated from a 454 (FLX) derived cDNA library of Boechera. RNA from live microdissected ovules and anthers at different developmental stages, as well as vegetative tissues of apomictic and sexual Boechera, were used to validate the candidates. SN - 1756-0500 UR - https://doi.org/10.1186/1756-0500-4-303 DO - 10.1186/1756-0500-4-303 ID - Pellino2011 ER -