Selection of reference genes for quantitative real-time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera

Table 2 Boechera accessions, including ovule and anther stage-specific developmental characteristics

Species^a	ID	Collection locality	Reproduction^b	Ovule-anther stage^c	Ovule development^d	Anther stage^g	Anther development^g
B. stricta	MT49	Sagebrush Meadow, MT	Sex	a	Nucellus	7-8	Premeiotic PMC
				b	MMC^e formation	9-10	Meiotic PMC^f
				c	Tetrad to degeneration	11	Microspore formation
				d	Fertilised ovules
B.divaricarpa	MT15	Vipond Park, MT	Apomixis	a	Nucellus	7-8	Premeiotic PMC
				b	MMC^e formation	9-10	Meiotic PMC
				c	Tetrad to degeneration	11	Microspore formation
				d	Fertilized ovules

^a Species identification were based upon silique orientation, trichome morphology, and cpDNA sequences [40]
^b Reproductive mode was confirmed using the flow cytometric seed screen [8, 41]
^c See Figures 1 and 2 for images of each stage.
^d According to Schneitz et al [38]
^e MMC: Megaspore mother cell
^f PMC: Pollen mother cell stage
^g According to Armstrong et al.[29]

ISSN: 1756-0500