Skip to main content

Table 3 Summary of the 2 best HKG combinations for different tissues and reproductive system according to geNorm

From: Selection of reference genes for quantitative real-time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera

Tissue Recommended HKGs M values V2/3 values Recommended HKGs (Boech RPS18 replaced by UBQ) M values V2/3 values
Apo all tissues Boech RPS18 Boech Efα1 0.130 0.042 Boech UBQ Boech Efα1 0.120 0.120
Apo vegetative tissues Boech Efα1 Boech ACT2 0.170 0.007 Boech UBQ Boech Efα1 0.220 0.120
Apo ovules Boech RPS18 Boech Efα1 0.060 0.040 Boech UBQ Boech Efα1 0.055 0.131
Apo anthers Boech ACT2 Boech Efα1 0.020 0.001 Boech ACT2 Boech Efα1 0.020 0.090
Sex all tissues Boech RPS18 Boech ACT2 0.017 0.078 Boech UBQ Boech ACT2 0.200 0.100
Sex vegetative tissues Boech RPS18 Boech Efα1 0.070 0.089 Boech UBQ Boech ACT2 0.220 0.100
Sex ovules Boech RPS18 Boech ACT2 0.010 0.088 Boech UBQ Boech Efα1 0.010 0.057
Sex anthers Boech Efα1 Boech ACT2 0.001 0.025 Boech Efα1 Boech ACT2 0.001 0.016