Workflow for the simultaneous extraction of DNA and RNA. The tissue punch is placed in 400 μl GTA buffer and vortexed until disrupted. The tissue is then completely homogenized by passing through a syringe (29G). The homogenate is then split for the purification of RNA and DNA. The split can be 1:1 or of different proportions depending on requirements of the experiment. The homogenate should be processed within a few hours.