Table 2 Yields of simultaneously isolated RNA and DNA from brain tissue punches (0,8 mm) of different brain regions and whole pituitary
From: Simultaneous DNA and RNA isolation from brain punches for epigenetics
Tissue | Total DNA yield [ng] | A260/280 | 260/230 | Total RNA yield [ng] | A260/280 | 260/230 |
|---|---|---|---|---|---|---|
PVN (n = 10) | 980 | 1,9 | 1,3 | 510 | 1,7 | 1 |
DG (n = 39) | 1165 | 1,9 | 1,2 | 275 | 1,9 | 1,1 |
Cortex (n = 9) | 675 | 1,9 | 1,4 | 330 | 1,9 | 1,2 |
Pituitary (n = 29) | 11400 | 1,9 | 1,9 | 1425 | 1,78 | 1,8 |