Table 1 The seven primer pairs used for HRM analyses and the corresponding amplification fragments.
From: Genetic screening of Fabry patients with EcoTILLING and HRM technology
Region | Primers | Amplicon sizes | Exon sizes | MgCl2 conc. |
|---|---|---|---|---|
Exon 1 | 5'-TCTTACGTGACTGATTATTGGTCT-3' 5'-CACACCCAAACACATGGAAA-3' | 416 bp | 254 bp | 3.5 mM |
Exon 2 | 5'-TGAAATCCCAAGGTGCCTAATA-3' 5'-GTACAGAAGTGCTTACAGTCC-3' | 314 bp | 174 bp | 3.5 mM |
Exon 3 | 5'-ACCTGGTGAAGTAACCTT-3' 5'-CTCAGCTACCATGGCCT-3' | 349 bp | 178 bp | 3.5 mM |
Exon 4 | 5'-GCTGGAAATTCATTTCTTTCCC-3' 5'-GGATGGTGAGAAGTGGTTG-3' | 285 bp | 91 bp | 3.5 mM |
Exon 5 | 5'-AATCTGTAAACTCAAGAGAAGGCTA-3' 5'-CTTTACCTGTATTTACCTTGAATG-3' | 349 bp | 161 bp | 3.5 mM |
Exon 6 | 5'-GATGCTGTGGAAAGTGGTT-3' 5'-GCCCAAGACAAAGTTGGTAT-3' | 355 bp | 198 bp | 3 mM |
Exon 7 | 5'-AGAATGAATGCCAAACTAAC-3' 5'-ATGAGCCACCTAGCCTTG-3' | 443 bp | 295 bp | 3.5 mM |