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Table 1 The seven primer pairs used for HRM analyses and the corresponding amplification fragments.

From: Genetic screening of Fabry patients with EcoTILLING and HRM technology

Region Primers Amplicon sizes Exon sizes MgCl2 conc.
Exon 1 5'-TCTTACGTGACTGATTATTGGTCT-3'
5'-CACACCCAAACACATGGAAA-3'
416 bp 254 bp 3.5 mM
Exon 2 5'-TGAAATCCCAAGGTGCCTAATA-3'
5'-GTACAGAAGTGCTTACAGTCC-3'
314 bp 174 bp 3.5 mM
Exon 3 5'-ACCTGGTGAAGTAACCTT-3'
5'-CTCAGCTACCATGGCCT-3'
349 bp 178 bp 3.5 mM
Exon 4 5'-GCTGGAAATTCATTTCTTTCCC-3'
5'-GGATGGTGAGAAGTGGTTG-3'
285 bp 91 bp 3.5 mM
Exon 5 5'-AATCTGTAAACTCAAGAGAAGGCTA-3'
5'-CTTTACCTGTATTTACCTTGAATG-3'
349 bp 161 bp 3.5 mM
Exon 6 5'-GATGCTGTGGAAAGTGGTT-3'
5'-GCCCAAGACAAAGTTGGTAT-3'
355 bp 198 bp 3 mM
Exon 7 5'-AGAATGAATGCCAAACTAAC-3'
5'-ATGAGCCACCTAGCCTTG-3'
443 bp 295 bp 3.5 mM
  1. The primers used in the EcoTILLING protocol were identical and differentiated by their marking with IRDye700 and IRDye800 in forward and reverse, respectively.