Table 2 Optimal reference genes for quantification of the entire dataset and individual (species, organs or stress) subsets
Program | Entire | Subsets | |||||||
|---|---|---|---|---|---|---|---|---|---|
Species | Organ | Stress | |||||||
A. stenosperma | A. hypogaea | A. duranensis | A. magna | Leaves | Roots | Biotic stress | Abiotic stress | ||
GeNorm (M) | ACT1/UBI1 (0.553) | ACT1/60S (0.269) | ACT1/UBI1 (0.535) | ACT1/UBI2 (0.350) | UBI2/60S (0.242) | ACT1/UBI1 (0.483) | UBI2/60S (0.492) | ACT1/60S (0.549) | UBI2/60S (0.376) |
NormFinder (M) | GAPDH (0.056) | ACT1 (0.062) | 60S (0.045) | 60S (0.057) | ACT2/PRO (0.013) | ACT1 (0.090) | GAPDH (0.063) | GAPDH (0.076) | GAPDH (0.091) |
BestKeeper (SD) | ACT1 (0.871) | 60S (0.284) | UBI2 (0.661) | EFA (0.677) | UBI1 (0.623) | UBI2 (0.603) | ACT1 (0.524) | ACT1 (0.945) | UBI1 (0.464) |