Figure 2

Representative whole-cell patch clamp recordings and current-voltage curves from SCCD epithelial cells grown on permeable supports. Left panel, Na⁺ -rich bath and pipette, Right panel, K⁺-rich bath and pipette. The holding potential and voltage command protocol are illustrated. Amiloride concentration was 100 μM. Bottom: Representative current-voltage relationships of the whole cell currents in the presence and absence of amiloride (100 μM). Voltages are corrected for the liquid junction potentials (see Methods).