Figure 1

Scheme of experimental design. Twenty-one candidate reference genes were evaluated for qPCR in peanut hairy root. Peanut nucleotide sequences were obtained using homologous sequences from Arabidopsis. Then, primer design was performed using AlleleID 7 software. The set of primers for the 21 candidate reference genes were tested in a pool of samples from peanut hairy roots. PCR efficiency was calculated and used as first screening to select the genes to be analyzed in two time course experiments in peanut hairy roots. Data analysis was performed to determine the best reference gene and optimal number of reference genes to be used for normalization procedures.