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Table 2 PCR efficiency of candidate reference genes

From: Selection of reference genes for qPCR in hairy root cultures of peanut

Target gene

Efficiencya

SE (E)b

Slopec

r2 d

NTCe

Reference gene ratingf

TUA3

116.3

0.037

-2.985

0.911

N/A

Poor

ACT7

99.1

0.012

-3.344

0.979

N/A

Excellent

TIP41

93.2

0.007

-3.497

0.991

N/A

Good

SAND

128.4

0.040

-2.787

9.190

N/A

Poor

H3

99.8

0.009

-3.328

0.987

N/A

Excellent

TBP2

107.2

0.026

-3.161

0.919

N/A

Good

RPB1

414.8

1.261

-1.405

0.218

N/A

Poor

EFα1

102.3

0.008

-3.268

0.991

N/A

Excellent

RPL8C

95.2

0.008

-3.442

0.990

N/A

Excellent

EIF4A1

107.0

0.017

-3.166

0.970

N/A

Excellent

CYP1

92.4

0.006

-3.519

0.993

N/A

Excellent

GAPDH

124.2

0.030

-2.852

0.925

N/A

To be considered

APT1

155.7

0.072

-2.453

0.884

N/A

Poor

UBQ11

114.9

0.018

-3.010

0.967

31.08

Poor (dimer)

AP47

86.8

0.008

-3.686

0.986

N/A

Poor

AT4G33380

83.5

0.028

-3.795

0.859

N/A

Poor

PP2AA3

120.7

0.051

-2.908

0.797

N/A

Poor

HEL

101.2

0.011

-3.293

0.983

N/A

Excellent

AT1G31300

99.9

0.008

-3.324

0.991

N/A

Excellent

rolC

105.2

0.012

-3.203

0.983

N/A

Good

aux1

602.4

0.568

-1.181

0.771

33.79

Poor

  1. a, (%) determines the performance of the PCR assay. b, Standard error of the efficiency. c, Slope calculated between log10RNA (ng/reaction) and Cq values. d, Determines the degree of linear correlation between log10RNA and Cq values. e, No template control. f, Observation was done based on efficiency values, NTC and melting curves analysis. Values were calculated using qbasePLUS.