Table 1 Characteristics of polymorphic SSR loci isolated from Picea abies L
From: Characterization of variable EST SSR markers for Norway spruce (Picea abies L.)
Locus name | GeneBank Accession | Repeat type | Primer Sequence (5' - 3') | Size range (bp) | Ta (°C) |
|---|---|---|---|---|---|
Pa_5 | GT887224 | (CCG)n | F: AGGTGGATATGTTCATCAAC | 136-158 | 53 |
| Â | Â | Â | R: CGCATACTCATCCCTAAC | Â | Â |
Pa_12 | AM170164 | (AAG)n | F: GACCGAGAACCCTTGTT | 136-138 | 53 |
| Â | Â | Â | R: CAAAAGCAGGAAAGAAGAAC | Â | Â |
Pa_22 | GT886979 | (TTC)n | F: TCACTGGCCACAGTTTATCG | 169-188 | 55 |
| Â | Â | Â | R: ATGAGGCCCAAGAGGAAGAC | Â | Â |
Pa_25 | GT887533 | (CTG)n | F: TGATTGAAATGATGGCTGCT | 131-165 | 55 |
| Â | Â | Â | R: CATGTACGGTGCTCCTCCTC | Â | Â |
Pa_28 | GT887962 | (TCG)n | F: GGCCGAAAGTGCTACTGCTA | 148-162 | 62 |
| Â | Â | Â | R: TGCTCCAGAAGAACACTCACA | Â | Â |
Pa_29 | GT884785 | (CAA)n | F: ACAACAGCAACAGCAGCAAC | 97-108 | 62 |
| Â | Â | Â | R: CGGGCTGAAGAATTTGTTGT | Â | Â |
Pa_33 | GT884592 | (CGG)n | F: GGTCGAGGAGGAGGAGGTAG | 91-104 | 62 |
| Â | Â | Â | R: CACCGCTAGTGCAGTCTCTG | Â | Â |
Pa_36 | GT887107 | (CGG)n | F: CGGCAGGAACATCACTGTTA | 178-200 | 62 |
| Â | Â | Â | R: ACCGTAACCTCCCCTACCAC | Â | Â |
Pa_41 | AM169657 | (CTG)n | F: CGAAGAAGAAAGCGAGGATG | 176-183 | 62 |
| Â | Â | Â | R: CAGGCTGCGAGAATCCTCTA | Â | Â |
Pa_42 | AM169827 | (CCG)n | F: CAATGCAATGGCCTCCTATC | 160-163 | 62 |
| Â | Â | Â | R: TAGACAGCAGCACGTCTCGT | Â | Â |
Pa_43 | AM169948 | (GAA)n | F: AACCAGCCGGAGTCTGTAAA | 168-175 | 62 |
| Â | Â | Â | R: TGCTTCTGTCTGACCAGGTG | Â | Â |
Pa_44 | AM171132 | (GGA)n | F: AAGGCAGCCAAAGTGAAGAA | 274-293 | 62 |
| Â | Â | Â | R: CTTGGCATTCCCTAGTGAGC | Â | Â |
Pa_47 | AM172144 | (CAG)n | F: ATCAATTGCCCTACCAGCAC | 106-118 | 62 |
| Â | Â | Â | R: TGCTCAATTTCCTGCATCTG | Â | Â |
Pa_48 | AM172164 | (CGG)n | F: ATTGCACAAGAGCGAACCTT | 131-195 | 62 |
| Â | Â | Â | R: CCAGCACCAAAATCACCAG | Â | Â |
Pa_49 | AM173566 | (ATG)n | F: GAAAGGCGAAAGCAAAACTG | 117,128 | 62 |
| Â | Â | Â | R: CCGTGTTTTTGAGGGAAGAA | Â | Â |
Pa_50 | AM174647 | (CAG)n | F: CCTCTCAGCCAAATGCAAAT | 124-129 | 62 |
| Â | Â | Â | R: GTCTCCGCAGAGCCATTACT | Â | Â |
Pa_51 | AM175215 | (CCA)n | F: CAGATGTGGGCACTTGTTTG | 119-140 | 62 |
| Â | Â | Â | R: TGGTCATGGTGGTGTTCAT | Â | Â |
Pa_52 | AM175251 | (CCA)n | F: GTAGTGTCACCGGCTCCTTT | 135-145 | 62 |
| Â | Â | Â | R: CTGGAGGATGATGGTGGTG | Â | Â |
Pa_53 | ES290836 | (TCG)n | F: CCCAATGAAGATCTCGATGC | 176-178 | 62 |
| Â | Â | Â | R: GGCTTTGCAGGAAACAAGAA | Â | Â |
Pa_55 | GT887161 | (GAA)n | F: TGAGCAACCAGAAAATCACG | 148-158 | 62 |
| Â | Â | Â | R: TCTCAACCCCTTTGTTTTCG | Â | Â |
Pa_56 | GT884695 | (AGGTG)n | F: ATCGTCTGCATTGCATTCAC | 113-128 | 62 |
| Â | Â | Â | R: CTTCGTTCCTTCCTGATCCA | Â | Â |
Pa_59 | AM169794 | (CTCTG)n | F: TAGACCACGTTGTGCTGAAA | 90-112 | 62 |
| Â | Â | Â | R: GCGACCTTTCGCAATTAAAC | Â | Â |
Pa_60 | GT885001 | (GGCTG)n | F: CGCCGTATCCATTCCCAAGC | 241-264 | 62 |
| Â | Â | Â | R: CCCAGCCCAGTTCAGTTTGC | Â | Â |