Internal control genes for quantitative RT-PCR expression analysis in mouse osteoblasts, osteoclasts and macrophages

Table 2 Candidate reference gene PCR primer sequences (5'-3'), amplicon sizes, PCR efficiencies and standard curve regression coefficients

Symbol	Forward primer	Reverse primer	Amplicon size (bp)	PCR efficiency (E)	Regression coefficient (R²)
18S	CTTAGAGGGACAAGTGGCG	ACGCTGAGCCAGTCAGTGTA	107	95%	0.9947
ACTB	CTCTGGCTCCTAGCACCATGAAGA	GTAAAACGCAGCTCAGTAACAGTCCG	200	94%	0.9975
B2M	CTGCTACGTAACACAGTTCCACCC	CATGATGCTTGATCACATGTCTCG	241	96%	0.9986
GAPDH	ACAGTCCATGCCATCACTGCC	GCCTGCTTCACCACCTTCTTG	266	95%	0.9981
HMBS	GAGTCTAGATGGCTCAGATAGCATGC	CCTACAGACCAGTTAGCGCACATC	250	94%	0.9962
HPRT1	GAGGAGTCCTGTTGATGTTGCCAG	GGCTGGCCTATAGGCTCATAGTGC	173	95%	0.9943

ISSN: 1756-0500