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Table 5 Statistics of non-chimeric and chimeric contigs >1 kb generated by the assemblies of unmasked and masked bcTi reads of pool 2 without separation by barcodes.

From: Sequencing of BAC pools by different next generation sequencing platforms and strategies

non-bc contigs

 

unmasked

m721

m361

number

non_chim2

354

461

562

 

chim2

328

239

199

total length (bp)

non_chim

2,912,280

2,888,385

3,024,333

 

chim

2,570,258

1,590,145

973,179

 

total

5,482,538

4,478,530

3,997,512

average length (bp)

non_chim

8,227

6,252

5,381

 

chim

7,836

6,681

4,890

fraction of total contig length

non_chim

0.53

0.64

0.76

 

chim

0.47

0.36

0.24

  1. 1 m72, m36: Reads were masked in regions where the 20mer frequency exceeds 72 and 36x, repectively.
  2. 2 Contigs composed by reads from a sole BAC with fractions <0.96 were regarded as chimeric (chim), all others as non-chimeric (non_chim).