Table 1 Sequencing and pyrosequencing primers used in this study.
From: Pyrosequencing assay for rapid identification of Mycobacterium tuberculosis complex species
Gene | Method | Primer name | Primer sequence (5' - 3') | Product size (bp) |
|---|---|---|---|---|
glpK | Sequencing | glpk-Forward | CGACCGCGACTTCCGCAAGT | 1,698 |
glpk-Reverse | AACATGTCCGCACGCTCGGG | |||
glpk-Internal 1 | TGATCCGCCGCAAGGCG | |||
glpk-Internal 2 | CTGGCGGCAAGCTGCAGT | |||
glpk-Internal 3 | GCTAAACCCGTGTACGCGCT | |||
glpk-Internal 4 | ATCGCGGTGACCGGCTC | |||
Pyrosequencing | glpk-573-F | AGAACGGCGACGCATTGT | 106 | |
glpk-573-R | Biotin- CTGGCGTTGGTTACATCGGT | |||
glpk-573-Seq | GGTGTTGTGGAATCTGA | |||
glpk-845-F | Biotin- GGGGAGGCGAAAAACACCTAT | 52 | ||
glpk-845-R | CGGTGTTCAGCAGCAGAAAA | |||
glpk-845-Seq | AGAAAATTGCCGGTC | |||
glpk-1379-F | TCACCGGCAACGACCTGT | 126 | ||
glpk-1379-R | Biotin- AGAACCCGACCGCCAAGC | |||
glpk-1379-Seq | GACCACCGCACTAGG | |||
pykA | Sequencing | pyka-Forward | TACCGCCGTCGCGACTATGC | 1,540 |
pyka-Reverse | TCGCAAGCGACCTGTTCACCG | |||
pyka-Internal 1 | CACGATCGGGTGTCCACC | |||
pyka-Internal 2 | TGGCCGGTGACCGGGTG | |||
pyka-Internal 3 | TCGATGGCGCCGACGCG | |||
pyka-Internal 4 | ATGCTGTCCGGGGAAACCT | |||
Pyrosequencing | pyka-Forward | GAACTGGTCCACGAGGTGA | 100 | |
pyka-Reverse | Biotin- GCACGATCGCTTCGAGATT | |||
pyka-Sequencing | CGGTGATCGCCAAGCT | |||
gyrB | Pyrosequencing | gyrB-6307-F | Biotin- CGCAAGCTACTGAAGGACAAGG | 113 |
gyrB-6307-R | TTGGTCTGGCCCTCGAACT | |||
gyrB-6307-seq | CCTTCACCGAGATCA | |||
gyrB-6406-F | AAGACCAAGTTGGGCAACAC | 121 | ||
gyrB-6406-R | Biotin- ACACAGCCTTGTTCACAACG | |||
gyrB-6406-seq | TGTGCAGAAGGTCTGTAA |