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Table 2 Mean( ± SEM) signal intensity, background intensity, number of detected probes and number of detected probe sets in hybridizations using 600 ng RNA from two NSCLC specimens (duplicates) each labeled using three different ATP-mix dilutions (1:50; 1:150 and 1:500).

From: Analytical variables influencing the performance of a miRNA based laboratory assay for prediction of relapse in stage I non-small cell lung cancer (NSCLC)

ATP-mix dilution

1:50

1:150

1:500

Mean Intensity

349 ± 27

339 ± 19

351 ± 9

Mean Background Intensity

89.3 ± 0.5

97.6 ± 4.6

92.3 ± 3.7

Number of Detected Probes

9762 ± 213

9378 ± 435

9585 ± 139

Number of Detected Probe Sets

2432 ± 28

2382 ± 101

2371 ± 12