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Table 5 Prognosticator calls (0 ="no recurrence" or 1 = "recurrence") were examined for varying RNA input amounts (a single T2 NSCLC specimen), ATP-mix dilutions (two NSCLC specimens), chip lot numbers (a single T2 NSCLC specimen), RNA extraction kits (four NSCLC specimens; HP = HighPure, Roche &RA = RecoverAll, Ambion) and RNA labeling kits (four NSCLC specimens; F = Flashtaq and FH = Flashtaq HSR, Genisphere) for selected samples using the prognostic profile of assay II.

From: Analytical variables influencing the performance of a miRNA based laboratory assay for prediction of relapse in stage I non-small cell lung cancer (NSCLC)

RNA amount Call Recurrence Chip lot Call Recurrence Labeling kit Call Recurrence
80 ng 1 - Lot x 1 - RPCI-18 - F1 1 No
100 ng 1 - Lot x 1 - RPCI-29 - F2 1 Yes
100 ng 1 - Lot y 1 - RPCI-57 - F3 1 No
100 ng 1 -     RPCI-64 - F4 1 No
160 ng 1 -       
400 ng 1 - RNA extraction kit    RPCI-18 - FH1 0 No
640 ng 1 -     RPCI-29 - FH2 0 Yes
    KH882A9 - HP 0 - RPCI-57 - FH3 0 No
ATP-mix dilution    KH22716D3 - HP 0 - RPCI-64 - FH4 0 No
    KH24218B4 - HP 0 -    
A-504; 1:50 0 - KH24935B5 - HP 0 -    
A-504; 1:150 0 -       
A-504; 1:500 0 - KH882A9 - RA 0 -    
B-652; 1:50 0 No KH22716D3 - RA 0 -    
B-652; 1:150 0 No KH24218B4 - RA 0 -    
B-652; 1:500 0 No KH24935B5 - RA 0 -    
  1. Data for recurrence was available only for the test of labeling kit and for one sample used to test ATP-mix dilution.