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Table 5 Prognosticator calls (0 ="no recurrence" or 1 = "recurrence") were examined for varying RNA input amounts (a single T2 NSCLC specimen), ATP-mix dilutions (two NSCLC specimens), chip lot numbers (a single T2 NSCLC specimen), RNA extraction kits (four NSCLC specimens; HP = HighPure, Roche &RA = RecoverAll, Ambion) and RNA labeling kits (four NSCLC specimens; F = Flashtaq and FH = Flashtaq HSR, Genisphere) for selected samples using the prognostic profile of assay II.

From: Analytical variables influencing the performance of a miRNA based laboratory assay for prediction of relapse in stage I non-small cell lung cancer (NSCLC)

RNA amount

Call

Recurrence

Chip lot

Call

Recurrence

Labeling kit

Call

Recurrence

80 ng

1

-

Lot x

1

-

RPCI-18 - F1

1

No

100 ng

1

-

Lot x

1

-

RPCI-29 - F2

1

Yes

100 ng

1

-

Lot y

1

-

RPCI-57 - F3

1

No

100 ng

1

-

   

RPCI-64 - F4

1

No

160 ng

1

-

      

400 ng

1

-

RNA extraction kit

  

RPCI-18 - FH1

0

No

640 ng

1

-

   

RPCI-29 - FH2

0

Yes

   

KH882A9 - HP

0

-

RPCI-57 - FH3

0

No

ATP-mix dilution

  

KH22716D3 - HP

0

-

RPCI-64 - FH4

0

No

   

KH24218B4 - HP

0

-

   

A-504; 1:50

0

-

KH24935B5 - HP

0

-

   

A-504; 1:150

0

-

      

A-504; 1:500

0

-

KH882A9 - RA

0

-

   

B-652; 1:50

0

No

KH22716D3 - RA

0

-

   

B-652; 1:150

0

No

KH24218B4 - RA

0

-

   

B-652; 1:500

0

No

KH24935B5 - RA

0

-

   
  1. Data for recurrence was available only for the test of labeling kit and for one sample used to test ATP-mix dilution.