Insights into N-calls of mitochondrial DNA sequencing using MitoChip v2.0

BMC Research Notes

Table 1 N-call analysis of mtDNA sequences using MitoChip v2.0 with Affymetrix GSEQ, sPROFILER, and ResqMi software.

Software	Means of N-Calls in rCRS (n = 16)	N-calls decrease %	P value	Total N-Calls	N-calls decrease %	P value
GSEQ	623			7212
ResqMi	407	35	p < 0.0005	5284	27	p < 0.0003
sPROFILER	239	61	p < 0.0003	5698	21	p < 0.0003

The results are shown as average of N-Call numbers and % decreases. P value calculated using the Wilcoxon signed rank test comparing the percentage drop in N-call using sPROFILER software and ResqMi software against Affymetrix GSEQ software. Total N-calls refer here to both MitoChip sections (rCRS and haplotypes).

ISSN: 1756-0500