Skip to main content

Table 2 Lengths of N-call stretches following mtDNA sequencing using MitoChip with GSEQ and sPROFILER.

From: Insights into N-calls of mitochondrial DNA sequencing using MitoChip v2.0

Sample Name

N-Call stretch lengths (bp)

Mutation found by conventional sequencing

 

*GSEQ

*sPROFILER

 

mtDNA1

5, 6, 9, 13

4, 5

1 point mutation (in 5 base stretch)

mtDNA2

5, 6, 12

4, 5, 6

1 point mutation (in 6 base stretch)

mtDNA3

5, 6, 7, 11

4, 10

1 point mutation, 1 insertion (in 10 base stretch)

mtDNA4

5, 6, 11

4, 5

1 insertion (in 5 base stretch)

mtDNA5

5, 13

4, 5

1 insertion (in 5 base stretch)

mtDNA6

5, 7, 11

4, 5, 11

1 point mutation, 1 insertion (in 11 base stretch)

mtDNA7

5, 10

4

Entire mtDNA sequenced: 1 point mutation

(in 4 N-call stretch)

mtDNA8

5, 6, 8, 9, 10, 11, 14, 18, 20

4, 5, 6, 8, 10,

18, 20

1 point mutation (in 20 base stretch)

2 point mutations (in 18 base stretch)

1 point mutation (in 10 base stretch)

mtDNA9

5, 6, 9, 11, 12

4, 5, 8

2 insertions (in 8 base stretch)

mtDNA10

5, 7, 8, 9, 10

4, 5, 7, 9

1 point mutation (in 9 base stretch)

mtDNA11

5, 6, 8, 9, 12

4, 5, 8

1 point mutation (in 8 base stretch)

mtDNA12

5, 9, 10

4, 9

1 insertion (in 9 base stretch)

mtDNA13

5, 7, 8, 9, 10

4, 5, 9

1 point mutation (in 9 base stretch)

mtDNA14

5, 8, 10

4, 5

No point mutation or indel (in 5 base stretch)

mtDNA15

5, 6, 7, 11

4, 5

No point mutation or indel (in 5 base stretch)

mtDNA16

5, 7, 8, 10, 11

4, 6

No point mutation or indel (in 6 base stretch)

  1. *Only stretches ≥ 4 bases identified in GSEQ are listed. For each sample only the longest N-call stretch was sequenced conventionally (except mtDNA7 and mtDNA8). Shorter N-call stretches occurred multiple times-as seen in Figure 1.