Skip to main content

Table 2 Results of BestKeeper, geNorm and NormFinder analyses in unstimulated and anti-CD3/CD28 stimulated T cells.

From: Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils

 

TBP

IPO8

SDHA

RPL13A

GAPDH

HBB

18S

GM [Cq]#

26.99

28.29

24.62

19.31

24.05

29.00

10.44

AM [Cq]#

26.99

28.30

24.63

19.31

24.08

29.02

10.46

min [Cq]#

25.99

27.23

23.58

18.76

22.09

26.60

9.35

max [Cq]#

27.91

29.36

25.76

20.25

25.52

30.24

11.22

SD [± Cq]#

0.45

0.52

0.55

0.40

0.81

0.94

0.58

CV [% Cq]#

1.68

1.82

2.22

2.07

3.35

3.25

5.53

M

0.941

0.873

1.035

0.793

1.203

1.508

0.920

S

0.168

0.142

0.204

0.083

0.252

0.384

0.169

  1. Expression stability of potential reference genes was calculated for n = 6 paired samples of unstimulated and anti-CD3/CD28 activated T cells.
  2. GM, geometric mean; AM, arithmetic mean; Cq, quantification cycle; SD, standard deviation; CV, coefficient of variation; #BestKeeper statistics; M, stability value determined by geNorm; S, stability value determined by NormFinder; expression stability decreases with increasing M and S values.