Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils

BMC Research Notes

Table 4 Results of BestKeeper, geNorm and NormFinder analyses in unstimulated and LPS-stimulated neutrophils.

	TBP	SDHA	18S	RPL13A	GAPDH	ACTB
GM [C_q]^#	29.27	30.03	11.94	28.60	24.54	20.37
AM [C_q]^#	29.28	30.06	11.96	28.64	24.57	20.40
min [C_q]^#	28.47	28.54	10.96	26.12	22.20	18.21
max [C_q]^#	30.80	33.17	13.60	31.63	26.54	21.80
SD [± C_q]^#	0.56	0.87	0.60	1.19	1.07	0.92
CV [% C_q]^#	1.90	2.90	5.05	4.14	4.35	4.53
M	0.810	0.879	0.969	0.968	0.935	0.800
S	0.170	0.204	0.229	0.230	0.218	0.155

Expression stability of potential reference genes was calculated for n = 7 paired samples of unstimulated and LPS-stimulated neutrophils.
GM, geometric mean; AM, arithmetic mean; C_q, quantification cycle; SD, standard deviation; CV, coefficient of variation; ^#BestKeeper statistics; M, stability value determined by geNorm; S, stability value determined by NormFinder; expression stability decreases with increasing M and S values.

ISSN: 1756-0500