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Table 5 Results of BestKeeper, geNorm and NormFinder analyses in total blood leukocytes (n = 12).

From: Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils

 

TBP

IPO8

SDHA

RPL13A

GAPDH

HBB

ACTB

18S

GM [Cq]#

28.45

29.33

27.84

20.39

23.23

17.55

20.34

11.17

AM [Cq]#

28.46

29.36

27.86

20.41

23.26

17.62

20.38

11.19

min [Cq]#

27.06

27.06

26.08

19.05

20.60

15.23

18.21

10.17

max [Cq]#

30.53

31.30

29.62

22.54

26.12

20.86

21.80

12.31

SD [± Cq]#

0.66

0.85

0.67

0.72

1.01

1.24

1.01

0.45

CV [% Cq]#

2.33

2.90

2.40

3.51

4.32

7.03

4.98

3.98

M

0.555

0.629

0.550

0.717

0.720

1.178

0.719

0.668

S

0.173

0.322

0.145

0.378

0.333

0.781

0.407

0.387

  1. GM, geometric mean; AM, arithmetic mean; Cq, quantification cycle; SD, standard deviation; CV, coefficient of variation; #BestKeeper statistics; M, stability value determined by geNorm; S, stability value determined by NormFinder; expression stability decreases with increasing M and S values.