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Table 1 Detection of PrPSc in Formalin Fixed Paraffin Embedded Tissues by ELISA.

From: PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA

TSE (species)

Animal #

Supernatant

Unenriched

Enriched

Scrapie (Sheep)

23

0.074

0.76

2.8

 

3503

0.11

0.61

2.40

 

3515

0.096

0.203

1.36

 

3740

0.056

0.19

0.73

 

3742

0.061

0.31

1.71

 

3506

0.064

0.42

1.27

Neg (Sheep)

0009

0.064

0.063

0.062

 

0007

0.083

0.067

0.074

 

3527

0.098

0.10

0.13

 

0050

0.077

0.059

0.062

 

0059

0.074

0.059

0.058

 

0004

0.089

0.031

0.089

CWD (WTD)

628

0.083

ND

0.459

 

632

0.08

ND

0.322

 

648

0.084

ND

0.971

 

654

0.086

ND

1.15

Neg (WTD)

645

0.085

ND

0.079

 

681

0.075

ND

0.080

TME (Cattle)

520

0.07

0.211

0.508

 

521

0.069

0.210

0.618

 

522

0.073

0.162

0.571

 

524

0.060

0.233

1.302

 

526

0.065

0.281

0.650

Neg (Cattle)

523

0.074

0.071

0.066

 

525

0.074

0.085

0.077

 

527

0.072

0.084

0.072

  1. An ELISA cutoff value of 0.18 is used throughout. The only incidence of an inoculated animal below the ELISA cutoff is shown in bold. Neg denotes a negative control animal.