Table 1 Summary of evaluation criteria for the RNA-isolation protocol
From: RNA isolation for transcriptomics of human and mouse small skin biopsies
Experiment | # | Biopsy weight* | RNA yield | RIN value | aRNA yield**$ | Array signal**$¥ | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
threshold | - | 0.1 μg Affymetrix 0.2 μg NimbleGen | 6.5 for both platforms | 10.0 μg Affymetrix 2.0 μg NimbleGen | - | ||||||
avg. | min. | avg. | min. | avg. | min. | avg. | min. | avg. | min. | ||
Human | 97 | 8.2 mg | 3.2 mg | 1.5 μg | 0.2 μg | 8.1 | 3.3 | 35.6 μg | 10.0 μg | 7.8 | 5.6 |
Mouse | 16 | ND | ND | 2.4 μg | 0.6 μg | 7.5 | 6.5 | 4.2 μg | 2.9 μg | 11.0 | 10.5 |