Figure 1

Schematic diagrams of five sul I vectors. (A) Plasmid map of the pK-Sul I construct; a pUC-derived vector that contains the CaMV double enhanced 35S promoter (CaMV d35S) fused to the sul I coding sequence with the CaMV 35S terminator (35St). (B) Map of the pCS binary vector containing the CaMV d35S-sul I-35St cassette derived from pK-SulI. (C) Map of the pCS4-BASK constitutive expression construct. In addition to the db35S driven sul I selection gene, the T-DNA contains the Solanum bulbocastanum 409s promoter (Sb Ubi409s), intron and first ubiquitin monomer coding sequence and a nos 3' terminator (nosT). A translational fusion to the ubiquitin monomer (Ubi) is required for overexpression. (D) Map of the pSUN binary vector which carries a nopaline synthase gene promoter (nosP) and terminator (nosT) to express the sul I selectable marker within the T-DNA. (E) Map of the pSUNG promoter testing construct, a pSUN-derived vector with a promoterless GUSPlus coding sequence and nos terminator located near the T-DNA right border. Unique and/or potentially useful restriction sites are shown. Gene coding sequences are drawn as blue arrows, promoters as green boxes, 3' terminators/poly adenylation signals as red boxes, introns as yellow boxes and the vector origin or replication sequences as gray boxes. Vector maps are not drawn to scale. Plasmid sequences are available from GenBank, accession numbers: HQ593859 - HQ593863.