Figure 2From: Novel sul I binary vectors enable an inexpensive foliar selection method in ArabidopsisSouthern blot analysis of pCS4-BASK ( sul I) transgenic plants. (A) Schematic representation of CS4-BASK T-DNA where Hind III, Bam HI and Spe I endonuclease sites are shown. The sul I hybridization probe is indicated by thick line. (B) Genomic DNA from seven randomly chosen sulfadiazine-resistant plants and a wildtype Col-0 plant were digested with Bam HI and hybridized with a 32P labeled sul I probe. The estimated T-DNA copy number in each plant is shown below the lane.Back to article page