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Table 1 Information on the primers used for real time PCR

From: Reference gene validation for qPCR in rat carotid body during postnatal development

Gene

Accession #

 

Primers

Product Size (bp)

Efficiency Rate (E)

PPIA

NM_017101

F

GTCAACCCCACCGTGTTCTTC

133

1.93

  

R

ATCCTTTCTCCCCAGTGCTCAG

  

TBP

NM_001004198

F

ACCGTGAATCTTGGCTGTAAAC

123

2.06

  

R

CGCAGTTGTTCGTGGCTCTC

  

RPII

AB017711

F

GGCTCTCCAGATTGCGATGTG

124

1.93

  

R

CAGGTAACGGCGAATGATGATG

  

β-actin

NM_031144

F

CAGGGTGTGATGGTGGGTATGG

115

2.03

  

R

AGTTGGTGACAATGCCGTGTTC

  

GAPDH

NM_017008

F

CAAGTTCAACGGCACAGTCAAG

123

1.91

  

R

ACATACTCAGCACCAGCATCAC

  

18s

X01117

F

CACGGGTGACGGGGAATCAG

105

2.03

  

R

CGGGTCGGGAGTGGGTAATTTG

  

TASK-1

NM_033376

F

GCAGAAGCCGCAGGAGTTG

126

2

  

R

GCCCGCACAGTTGGAGATTTAG

  

TASK-2

AM229406

F

ACGCCCTCTACCGCTACTTTG

129

2.12

  

R

GCCGCCTCCTCCTCTTCTTG

  

TASK-3

NM_053405

F

CGGTGCCTTCCTCAATCTTGTG

144

1.8

  

R

TGGTGCCTCTTGCGACTCTG

  
  1. Nucleotide sequences for the primers, size of PCR products, and PCR amplification efficiency rate of each primer set. All the genes are from rat origin. The real-time PCR efficiency rate (E) in exponential phase was calculated according to the equation: E = 10[-1/slope] [32].