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Table 2 Ranking of the 6 selected reference genes in rat whole carotid bodies by geNorm, NormFinder and BestKeeper

From: Reference gene validation for qPCR in rat carotid body during postnatal development

geNorm N1+N14 (n = 16) N14+Hyper14 (n = 14) N14+Hypo14 (n = 14) N1+N14+Hyper14+ Hypo14 (n = 28)
Rank Gene M-value Gene M-value Gene M-value Gene M-value
Best two genes RPII TBP 0.26 PPIA TBP 0.20 PPIA TBP 0.29 PPIA TBP 0.26
3 PPIA 0.27 RPII 0.35 RPII 0.36 RPII 0.38
4 actin 0.34 GAPDH 0.43 GAPDH 0.45 GAPDH 0.44
5 GAPDH 0.38 actin 0.50 actin 0.50 actin 0.49
6 18s 0.40 18s 0.57 18s 0.52 18s 0.53
Norm Finder N1+N14 (n = 16) N14+Hyper14 (n = 14) N14+Hypo14 (n = 14) N1+ N14+Hyper14+ Hypo14 (n = 28)
Rank Gene Variability Gene Variability Gene Variability Gene Variability
Best two genes TBP Actin 0.009 RPII GAPDH 0.007 RPII GAPDH 0.008 PPIA TBP 0.011
1 GAPDH 0.01 RPII 0.013 TBP 0.007 TBP 0.015
2 PPIA 0.011 GAPDH 0.013 PPIA 0.015 GAPDH 0.017
3 actin 0.014 TBP 0.014 GAPDH 0.018 18s 0.019
4 TBP 0.015 actin 0.019 RPII 0.018 PPIA 0.020
5 RPII 0.016 PPIA 0.021 18s 0.020 RPII 0.021
6 18s 0.018 18s 0.021 actin 0.021 actin 0.023
Best Keeper N1+N14 (n = 16) N14+ yper14 (n = 14) N14+Hypo14 (n = 14) N1 + N14 + Hyper14+ Hypo14 (n = 28)
Rank Gene Coeff. of corr.[r] Gene Coeff. of corr.[r] Gene Coeff. of corr.[r] Gene Coeff. of corr.[r]
1 PPIA 0.89 RPII 0.97 RPII 0.86 PPIA 0.97
2 actin 0.84 PPIA 0.96 PPIA 0.85 TBP 0.95
3 GAPDH 0.83 GAPDH 0.96 TBP 0.85 GAPDH 0.95
4 18s 0.73 TBP 0.95 actin 0.75 RPII 0.93
5 RPII 0.72 actin 0.93 GAPDH 0.69 actin 0.92
6 TBP 0.70 18s 0.87 18s 0.56 18s 0.89
  1. The reference genes are ranked in several groups of CB as follows: N1+N14 = immature and mature CB, reared in normoxia (n = 16). N14+Hyper14 = mature normoxia and hyperoxia treated CB (n = 14). N14+Hypo14 = mature normoxia and hypoxia treated CB (n = 14). N1+N14+Hyper14+Hypo14 = immature, normoxia (N1), mature, normoxia (N14), mature, hyperoxia (Hyper14), and mature, hypoxia treated (Hypo14) CB (n = 28). Genes are ranked on their stability value calculated by each respective program: geNorm, M-value; NormFinder, Variability; and BestKeeper, Coefficient of correlation [r]. (n) = number of independent determinations.