Skip to main content

Table 3 Consensus ranking

From: Reference gene validation for qPCR in rat carotid body during postnatal development

Gene ranking for group N1+N14 (n = 16)
Rank Position geNorm NormFinder BestKeeper Consensus
1 RPII GAPDH PPIA PPIA
2 TBP PPIA Actin GAPDH
3 PPIA Actin GAPDH Actin
4 Actin TBP 18s RPII
5 GAPDH RPII RPII TBP
6 18s 18s TBP 18s
Gene ranking for group N14+Hyper14 (n = 14)
Rank Position geNorm NormFinder BestKeeper Consensus
1 PPIA RPII RPII RPII
2 TBP GAPDH PPIA TBP
3 RPII TBP GAPDH GAPDH
4 GAPDH Actin TBP PPIA
5 Actin PPIA Actin Actin
6 18s 18s 18s 18s
Gene ranking for group N14+Hypo14 (n = 14)
Rank Position geNorm NormFinder BestKeeper Consensus
1 PPIA TBP RPII TBP
2 TBP PPIA PPIA PPIA
3 RPII GAPDH TBP RPII
4 GAPDH RPII Actin GAPDH
5 Actin 18s GAPDH Actin
6 18s Actin 18s 18s
Gene ranking for group N1+N14+Hyper14+Hypo14 (n = 28)
Rank Position geNorm NormFinder BestKeeper Consensus
1 PPIA TBP PPIA TBP
2 TBP GAPDH TBP PPIA
3 RPII 18s GAPDH GAPDH
4 GAPDH PPIA RPII RPII
5 Actin RPII Actin 18s
6 18s Actin 18s Actin
  1. Weighted rank aggregation was performed to combine the ordered lists of genes produced by geNorm, NormFinder, and BestKeeper to a consensus rank of genes.