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Figure 4 | BMC Research Notes

Figure 4

From: Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues

Figure 4

Determination of the optimal number of reference genes for normalization . The geNorm software calculates the normalization factor from an increasing number of genes (starting with at least two) for which the variable V defines the pairwise variation between two sequential normalization factors. The lower the pairwise variation, the better is the combination of genes for reference. V6/7 for example, shows the variation between the normalization factors of six genes in relation to seven genes and shows that six genes is the combination providing the lowest pairwise variation.

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