Figure 3
From: The C313Y Piedmontese mutation decreases myostatin covalent dimerisation and stability
Myostatin precursor protein disulphide mutants are structurally similar to the WT but have decreased thermal stability. A. Circular dichroism spectra from 195 to 240 nm of MstnPP (green), C313Y-MstnPP (blue), C313A-MstnPP (black) and C374A-MstnPP (red). B. Fluorescence-based thermal shift assay; MstnPP (green); C313Y-MstnPP (blue); C313A-MstnPP (black); C374A-MstnPP (red); and buffer only control (orange).