Furin proteolytic processing of C313Y-MstnPP and C313Y-MstnGF secondary structure is maintained. A. Analysis of (i) WT and (ii) C313Y precursor protein furin cleavage products by reducing (R) and non-reducing (NR) 15% SDS-PAGE. WT growth factor dimer (**) and C313Y growth factor monomer (*) under non-reducing conditions are indicated. B. Circular dichroism analysis of MstnLC and C313Y-MstnLC from 195 to 240 nm. (i) Comparison of C313Y-MstnPP (blue) and C313Y-MstnLC (black). (ii) Comparison of WT MstnPP (green) and MstnLC (red). (iii) Comparison of C313Y-MstnLC (black) and MstnLC (red).