Expression of IL-1β, HIF-1α, GAPDH, and PPIA in the air pouch membrane in a 50-hour time course of MSU crystal inflammation. A. Leukocyte numbers were determined in lavaged pouch exudates by manual cell counting with a hemocytometer. Values are expressed as 1 × 106 leukocytes/pouch (y-axis). B. Changes in expression of IL-1β, HIF-1α, GAPDH and PPIA relative to t = 0h. Four mice were used at t = 1h and t = 4h, and 3 mice at each of the other time points. For each target, the p value (t test) is indicated at the time point of maximal fold change with respect to t = 0h.