Figure 2

BdcA increases swarming and swimming motility. (A) Swarming motility of P. aeruginosa PA14 and R. meliloti 102F34 with BdcA production. The P. aeruginosa BM-2 plates were incubated at 37°C with 250 μg/mL chloramphenicol for 15 h, and the R. meliloti Eiken agar plates were incubated at 30°C with 50 μg/mL chloramphenicol for 15 h. (B) Swimming motility of P. aeruginosa, P. fluorescens, and R. meliloti 102F34. The P. aeruginosa plates were incubated at 37°C with 250 μg/mL chloramphenicol for 11 h, the P. fluorescens plates were incubated at 30°C with 250 μg/mL chloramphenicol for 20 h, and the R. meliloti plates were incubated at 30°C with 50 μg/mL chloramphenicol for 15 h. Black bars show the motility for strains expressing bdcA via pMMB206-BdcA and the gray bars indicate the control strains with pMMB206. For each strain, 0.1 mM IPTG was added to induce bdcA expression. ** indicates statistical significant difference as determined by a Student's t-test (p < 0.05). Error bars indicate the standard deviation of two independent cultures.