BdcA disperses P. aeruginosa and R. meliloti biofilms in flow cell experiments. Representative IMARIS images of P. aeruginosa/pMMB206-BdcA/pP25-gfp and P. aeruginosa/pMMB206/pP25-gfp flow cell biofilm formation after 72 h and 120 h of incubation with LB medium at 37°C (A). IMARIS images of R. meliloti/pMMB206-BdcA/pP25-gfp and R. meliloti/pMMB206/pP25-gfp flow cell biofilm formation after 67 h and 115 h of incubation with LB medium at 30°C (B). After forming biofilms in LB for 24 h, IPTG (0.1 mM) was added to induce BdcA production from pMMB206-BdcA. Each strain has pP25-gfp for producing GFP to visualize the biofilms, and carbenicillin (50 μg/mL) was added to retain pP25-gfp. Chloramphenicol (250 μg/mL for P. aeruginosa and 50 μg/mL for R. meliloti) was used to retain the pMMB206-based plasmids. Scale bars represent 10 μm.