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Table 1 Strains and plasmids used in this study

From: Escherichia coli BdcA controls biofilm dispersal in Pseudomonas aeruginosa and Rhizobium meliloti

Strain/Plasmid Genotype Source
Strain
E. coli BW25113 lacI q rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 [34]
E. coli DH5α luxS supE44 ΔlacU169(Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [21, 35]
E. coli DH5α/ pMMB206/pRK2013 E. coli harboring pMMB206 and pRK2013 This study
E. coli DH5α/ pMMB206-BdcA/pRK2013 E. coli harboring pMMB206-BdcA and pRK2013 This study
P. aeruginosa PA14 P. aeruginosa wild-type strain [36]
P. fluorescens P. fluorescens wild-type strain R. Frazee
R. meliloti 102F34 R. meliloti wild-type strain M. Sadowski
PA14/pMMB206 P. aeruginosa wild-type harboring pMMB206 This study
PA14/pMMB206-BdcA P. aeruginosa wild-type harboring pMMB206-BdcA This study
PA14/ pMMB206/pP25-gfp P. aeruginosa wild-type harboring pMMB206 and pP25-gfp This study
PA14/ pMMB206-BdcA/pP25-gfp P. aeruginosa wild-type harboring pMMB206-BdcA and pP25-gfp This study
P. fluorescens/pMMB206 P. fluorescens wild-type harboring pMMB206 This study
P. fluorescens/pMMB206-BdcA P. fluorescens wild-type harboring pMMB206-BdcA This study
R. meliloti 102F34/pMMB206 R. meliloti wild-type harboring pMMB206 This study
R. meliloti 102F34/pMMB206-BdcA R. meliloti wild-type harboring pMMB206-BdcA This study
R. meliloti 102F34/pMMB206/pP25-gfp R. meliloti wild-type harboring pMMB206 and pP25-gfp This study
R. meliloti 102F34/pMMB206-BdcA/pP25-gfp R. meliloti wild-type harboring the pMMB206-BdcA and pP25-gfp This study
Plasmid
pMMB206 Cmr, Mob+, lacIq, broad-host range plasmid [23]
pMMB206-BdcA Cmr; pMMB206 with P tac-lacUV5 ::bdcA this work
pRK2013 Kmr; Tra+ Mob+ (RK2) Km::Tn7 ColEl origin, helper plasmid for mobilization M. Bagdasarian, [37]
pP25-gfp Carr; constitutive green fluorescent protein production [27]
  1. Cmr, Carr, and Kmr, denote chloramphenicol, carbenicillin, and kanamycin resistance, respectively.