Escherichia coli BdcA controls biofilm dispersal in Pseudomonas aeruginosa and Rhizobium meliloti

BMC Research Notes

Table 2 Flow cell statistical analysis of biofilm formation via COMSTAT

Time	Biomass (μm³/μm²)	Surface coverage (%)	Average thickness (μm)	Roughness	Biomass (μm³/μm²)	Surface coverage (%)	Average thickness (μm)	Roughness
	*P. aeruginosa* /pMMB206/pP25-gfp				*P. aeruginosa* /pMMB206-BdcA/pP25-gfp
72 h	3 ± 1	36 ± 7	6 ± 3	0.5 ± 0.3	4 ± 1	37 ± 9	8 ± 3	0.5 ± 0.2
120 h	3.1 ± 0.9	37 ± 8	7 ± 3	0.5 ± 0.2	0.2 ± 0.1	13 ± 4	0.5 ± 0.4	1.6 ± 0.2
	*R. meliloti* /pMMB206/pP25-gfp				*R. meliloti* /pMMB206-BdcA/pP25-gfp
67 h	3 ± 1	34 ± 8	6 ± 2	0.6 ± 0.3	3 ± 1	40 ± 10	6 ± 2	0.7 ± 0.2
115 h	2.8 ± 0.7	30 ± 6	6 ± 2	0.5 ± 0.1	0.4 ± 0.3	17 ± 5	0.9 ± 0.7	1.3 ± 0.3

For P. aeruginosa, cultures were grown in LB with 250 μg/mL chloramphenicol and 50 μg/mL carbenicillin for 24 h, then bdcA expression was induced by adding 0.1 mM IPTG. Data were collected at 72 h (48 h after IPTG addition) and 120 h (96 h after IPTG addition). For R. meliloti, cultures were grown in LB with 50 μg/mL chloramphenicol and 50 μg/mL carbenicillin for 24 h, then bdcA expression was induced by adding 0.1 mM IPTG. Data were collected at 67 h (43 h after IPTG addition) and 115 h (91 h after IPTG addition). Data are the average of two independent cultures for each strain.

ISSN: 1756-0500