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Table 2 Flow cell statistical analysis of biofilm formation via COMSTAT

From: Escherichia coli BdcA controls biofilm dispersal in Pseudomonas aeruginosa and Rhizobium meliloti

Time Biomass
(μm3/μm2)
Surface coverage
(%)
Average thickness
(μm)
Roughness Biomass
(μm3/μm2)
Surface coverage
(%)
Average thickness
(μm)
Roughness
  P. aeruginosa /pMMB206/pP25-gfp P. aeruginosa /pMMB206-BdcA/pP25-gfp
72 h 3 ± 1 36 ± 7 6 ± 3 0.5 ± 0.3 4 ± 1 37 ± 9 8 ± 3 0.5 ± 0.2
120 h 3.1 ± 0.9 37 ± 8 7 ± 3 0.5 ± 0.2 0.2 ± 0.1 13 ± 4 0.5 ± 0.4 1.6 ± 0.2
  R. meliloti /pMMB206/pP25-gfp R. meliloti /pMMB206-BdcA/pP25-gfp
67 h 3 ± 1 34 ± 8 6 ± 2 0.6 ± 0.3 3 ± 1 40 ± 10 6 ± 2 0.7 ± 0.2
115 h 2.8 ± 0.7 30 ± 6 6 ± 2 0.5 ± 0.1 0.4 ± 0.3 17 ± 5 0.9 ± 0.7 1.3 ± 0.3
  1. For P. aeruginosa, cultures were grown in LB with 250 μg/mL chloramphenicol and 50 μg/mL carbenicillin for 24 h, then bdcA expression was induced by adding 0.1 mM IPTG. Data were collected at 72 h (48 h after IPTG addition) and 120 h (96 h after IPTG addition). For R. meliloti, cultures were grown in LB with 50 μg/mL chloramphenicol and 50 μg/mL carbenicillin for 24 h, then bdcA expression was induced by adding 0.1 mM IPTG. Data were collected at 67 h (43 h after IPTG addition) and 115 h (91 h after IPTG addition). Data are the average of two independent cultures for each strain.