Figure 1From: Saccharomyces cerevisiae chitin biosynthesis activation by N-acetylchitooses depends on size and structure of chito-oligosaccharidesThin layer chromatography analysis of [14C]-chitin, produced in vitro by S. cerevisiae membrane fractionation. The CHS reaction product was digested by S. Griseus chitinase and analysed on silica 60 TLC plates as described in Materials and Methods. Samples were loaded onto the TLC plate before (panel A) or after 24 h incubation with commercial chitinase (panel B). In panel C the reference sample containing commercial [14C]-GlcNAc was applied.Back to article page