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Table 1 Per cent activation of [14C]-GlcNAc incorporation into chitin polymer in presence of oligosaccharides from different lengths and structures.

From: Saccharomyces cerevisiae chitin biosynthesis activation by N-acetylchitooses depends on size and structure of chito-oligosaccharides

DP N-acetyl-chitooses
β-(1,4)-Glc-NAc
Laminari-oses
β-(1,3)-Glc
Dextro-oses
α-(1,6)-Glc
Cello-oses
β-(1,4)-Glc
2 13,3% ± 2 2% ± 1,1 nd nd
3 10% ± 1,5 1,6% ± 1,2 2% ± 1,3 nd
4 17,3% ± 0,8 0,5% ± 0,5 1,5% ± 1,2 nd
5 23,3% ± 1,1 1% ± 0,8 2,1% ± 1,5 3% ± 2
8 24,4% ± 1,2 nd nd nd
  1. Chitin synthase activity of S. cerevisiae membrane fractions was measured as incorporation of [14C]-GlcNAc into a filter-retained insoluble material. Assays were performed upon preincubation of microsomes with N-acetylchitooses β-(1,4)-GlcNAc, laminarioses β-(1,3)-Glc, dextrooses α-(1,6)-Glc, or cellooses β-(1,4)-Glc. Length of oligosaccharides tested varied from bioses to octaoses (DP = degree of polymerization). Per cent activations were calculated compared to standard assay without added oligosaccharides. Results are the average of five independent experiments (±SD). Activation for some oligosaccharides was not determined (nd).