Figure 4From: Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genesHomology models of H. coronopifolia defensins depicting the amino acid differences observed in the alignment of the deduced amino acid sequences. (A) Hc-AFP1, (B) Hc-AFP3, (D) Hc-AFP2, (E ) Hc-AFP4, (C and F) RMSD analysis of Hc-AFP1 and 3 and Hc-AFP2 and 4 respectively. (G) Structural alignment of the backbones of Hc-AFP1-4 (H) RMSD analysis of the structural alignment of Hc-AFP1-4. Models were created using Rs-AFP1 (Protein Data Bank: 1AYJ) as template. Acidic residues are indicated in red, basic in blue and polar residues in green. The black arrows indicate significant differences in structure between Hc-AFP1 and 3 (Group 1) and Hc-AFP2 and 4 (Group 2). The green arrow indicates the difference in presentation of the loop connecting β-strand 2 and 3.Back to article page