Homology models of H. coronopifolia defensins depicting the amino acid differences observed in the alignment of the deduced amino acid sequences. (A) Hc-AFP1, (B) Hc-AFP3, (D) Hc-AFP2, (E ) Hc-AFP4, (C and F) RMSD analysis of Hc-AFP1 and 3 and Hc-AFP2 and 4 respectively. (G) Structural alignment of the backbones of Hc-AFP1-4 (H) RMSD analysis of the structural alignment of Hc-AFP1-4. Models were created using Rs-AFP1 (Protein Data Bank: 1AYJ) as template. Acidic residues are indicated in red, basic in blue and polar residues in green. The black arrows indicate significant differences in structure between Hc-AFP1 and 3 (Group 1) and Hc-AFP2 and 4 (Group 2). The green arrow indicates the difference in presentation of the loop connecting β-strand 2 and 3.