Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes

Table 3 Primers used in the q-RT-PCR analysis of the Hc-AFP defensin genes

Primer set	Sequence 5'→3'	Primer	Target gene	PCR eff.
Helio EF Fw Helio EF Rv	ATGGGTAAAGAGAAGTTTCACATCAA GTTGGGTCCTTCTTGTCAACACTC	150 nm 200 nm	H. coronopifolia elongation factor 1α	0.99
Hc-AFP1 Rt Fw Hc-AFP1 Rt Rv	TCAGGAGTTTGTGGAAACAGTGG GCAGCCAACATAAACATATTTTGGA	200 nm 150 nm	Hc-AFP1	0.98
Hc-AFP2 Rt Fw Hc-AFP2 Rt Rv	CGTGTAGGAACCAGTGCATCAAC TAGGATTTTTCTGGTATGGCCG	150 nm 200 nm	Hc-AFP2	0.99
Hc-AFP3 Rt Fw Hc-AFP3 Rt Rv	TCAGGAGTTTGTGGAAACACTGA ATCATTAGAAGCTGCCAACATAAACTAG	150 nm 200 nm	Hc-AFP3	0.97
Hc-AFP4 Rt Fw Hc-AFP4 Rt Rv	ATGGTGGAAGCTCAGAAGTTGTGT GCTAGCAGCAAAGATGTTTGTTTG	200 nm 150 nm	Hc-AFP4	0.92

ISSN: 1756-0500